Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 cells
antibody
input
genotype/variation
Dicer2 kd rescue CHARRs
cell line
S2 cells
passages
E20-24h
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP sequencing, DNA libraries were constructed using the NEBNext® UltraTM II DNA Library Prep kit (NEB, USA) following manufacturer's recommendations. After end Repair, 5 ́ phosphorylation and dA-Tailing of purified DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated and he library fragments were purified with SPRIselect sample purification beads (NEB, USA). At last, PCR products were purified, and library quality was assessed on the Agilent Bioanalyzer 2100 system. The libraries were sequenced at the Annoroad Gene Technology (Beijing, China) on an Illumina HiSeq X Ten platform and 150 bp paired-end reads were generated.

Sequencing Platform

instrument_model
Illumina HiSeq X Ten

dm6

Number of total reads
4572222
Reads aligned (%)
96.7
Duplicates removed (%)
9.8
Number of peaks
1415 (qval < 1E-05)

dm3

Number of total reads
4572222
Reads aligned (%)
98.0
Duplicates removed (%)
10.2
Number of peaks
1298 (qval < 1E-05)

Base call quality data from DBCLS SRA