Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
293T-Rex
cell line
293T-Rex
transgene
N3 (empty vector), no dox
epitope tag
Flag-HA-OneSTrEP (N-terminal)
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared according to manufacturer’s instructions (Illumina). Immunoprecipitated DNA was first end-repaired using End-It Repair Kit (Epicenter), tailed with an A using Klenow exo minus (NEB M0212), and ligated to custom adapters with LigaFast (Promega, city, state #M8225). Fragments of 300±50 bp were size-selected and subjected to ligation-mediated PCR amplification (LM-PCR), using Phusion DNA polymerase (NEB M0530).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
20972614
Reads aligned (%)
92.2
Duplicates removed (%)
14.5
Number of peaks
1222 (qval < 1E-05)

hg19

Number of total reads
20972614
Reads aligned (%)
91.0
Duplicates removed (%)
16.7
Number of peaks
1246 (qval < 1E-05)

Base call quality data from DBCLS SRA