GSM3940467: Dam SG SetDB1-KO 1; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Larvae
Cell type
Salivary glands
NA
NA
Attributes by original data submitter
Sample
source_name
salivary glands
genotype
SetDB1 mutant
developmental stage
L3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
50 egg chambers were dissected for each sample. DNA was isolated using phenol-chloroform method. 300ng of genomic DNA was digested by DpnI endonuclease. Digested DNA was ligated with double-stranded DNA adapters and then digested with DpnII endonuclease. Next, methylated fragments were selectively amplified using adapter-specific primer. Before the preparation of libraries for Illumina DamID-seq, the adapters used in DamID procedure were cut with DpnII enzyme. No further fragmentation was performed.