Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Larvae
Cell type
Salivary glands
NA
NA

Attributes by original data submitter

Sample

source_name
salivary glands
genotype
SetDB1 mutant
developmental stage
L3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
50 egg chambers were dissected for each sample. DNA was isolated using phenol-chloroform method. 300ng of genomic DNA was digested by DpnI endonuclease. Digested DNA was ligated with double-stranded DNA adapters and then digested with DpnII endonuclease. Next, methylated fragments were selectively amplified using adapter-specific primer. Before the preparation of libraries for Illumina DamID-seq, the adapters used in DamID procedure were cut with DpnII enzyme. No further fragmentation was performed.

Sequencing Platform

instrument_model
Illumina MiSeq

dm6

Number of total reads
1390714
Reads aligned (%)
86.0
Duplicates removed (%)
68.8
Number of peaks
86 (qval < 1E-05)

dm3

Number of total reads
1390714
Reads aligned (%)
86.0
Duplicates removed (%)
68.4
Number of peaks
141 (qval < 1E-05)

Base call quality data from DBCLS SRA