Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PPP1R10

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293+pCW57 bla Avi-PNUTS WT
cell line
HEK293
growth conditions
DMEM 10% FCS, +blasticidin, +doxycycline
antibody
Rabbit anti-avi-tag (GenScript A00674)
treatment
PNUTS expression was induced with 2ug/ml doxycycline for 24 hrs,

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sonicated with a Bioruptor or Covaris Ultrasonicator 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, SPRI bead purification of ~200bp fragments, and amplification (15-18 cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg19

Number of total reads
24602487
Reads aligned (%)
118.6
Duplicates removed (%)
13.8
Number of peaks
588 (qval < 1E-05)

hg38

Number of total reads
24602487
Reads aligned (%)
119.6
Duplicates removed (%)
13.7
Number of peaks
745 (qval < 1E-05)

Base call quality data from DBCLS SRA