GSM3938732: 293 pCW57 PNUTSW401A Dox Spt5PO4 Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SUPT5H
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293+pCW57 bla Avi-PNUTS W401A
cell line
HEK293
growth conditions
DMEM 10% FCS, +blasticidin, +doxycycline
antibody
rabbit anti Spt5 (Glover-Cutter et al NSMB 15:71-78, 2008)
treatment
PNUTS W401A expression was induced with 2ug/ml doxycycline for 24 hrs,
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sonicated with a Bioruptor or Covaris Ultrasonicator 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, SPRI bead purification of ~200bp fragments, and amplification (15-18 cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.