Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SUPT5H

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293 Flp-in T-REx pcDNA5 HA-NS1A
cell line
HEK293
growth conditions
DMEM 10% FCS, +hygromycin, +blasticidin
antibody
rabbit anti Spt5 (Glover-Cutter et al NSMB 15:71-78, 2008)
treatment
control without doxycycline induction

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sonicated with a Bioruptor or Covaris Ultrasonicator 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, SPRI bead purification of ~200bp fragments, and amplification (15-18 cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
12711877
Reads aligned (%)
63.8
Duplicates removed (%)
7.3
Number of peaks
9949 (qval < 1E-05)

hg38

Number of total reads
12711877
Reads aligned (%)
64.4
Duplicates removed (%)
7.0
Number of peaks
10019 (qval < 1E-05)

Base call quality data from DBCLS SRA