GSM3938709: Hap1 PSMG1 pA-MT PolII Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
HAP1
NA
NA
Attributes by original data submitter
Sample
source_name
Hap1-PSMG1-pA-MT
cell line
Hap1
growth conditions
IMDM 10% FCS,
antibody
Rabbit anti pan CTD (Schroeder et al Genes Dev. 14:2435, 2000)
treatment
no treatment
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sonicated with a Bioruptor or Covaris Ultrasonicator 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, SPRI bead purification of ~200bp fragments, and amplification (15-18 cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.