GSM3938577: IMR90 EMPTY GM input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
IMR90
cell type
Lung fibroblast
ectopic gene expression
EMPTY VECTOR
passage from isolation
passage 21-28
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIPSeq lysates were clarified from sonicated crosslinked nuclei and H3K27ac-DNA complexes were isolated with antibody. ChIPSeq libraries were prepared as in http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf.