Antigen

Antigen Class

TFs and others

Antigen

TOP2B

Cell type

Cell type Class

Neural

Cell type

0777A

NA

NA

Sample

source_name

0777A glioma cells

agent

DMSO

cell line

0777A

cell type

glioma cells

chip antibody

TOP2B

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear extracts were isolated, chromatin fragmented using Covaris sonicator in 1mL tubes for 1min. 10 ug of antibody (TOP2A Abcam, TOP2B Abcam) was incubated overnight. Washes with RIPA Buffer 4 times and once of TE with 50mM NaCl. Reversed crosslink and DNA purified for sequencing using phenol:chloroform:isoamyl alcohol.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

16849662

Reads aligned (%)

53.4

Duplicates removed (%)

66.1

Number of peaks

2 (qval < 1E-05)

hg19

Number of total reads

16849662

Reads aligned (%)

53.3

Duplicates removed (%)

66.7

Number of peaks

4 (qval < 1E-05)