GSM3938472: TOP2B SNB19 ETO chip-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TOP2B
Cell type
Cell type Class
Neural
Cell type
SNB-19
Primary Tissue
Brain
Tissue Diagnosis
Astrocytoma
Attributes by original data submitter
Sample
source_name
SNB19 glioma cells
agent
ETO
cell line
SNB19
cell type
glioma cells
chip antibody
TOP2B
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear extracts were isolated, chromatin fragmented using Covaris sonicator in 1mL tubes for 1min. 10 ug of antibody (TOP2A Abcam, TOP2B Abcam) was incubated overnight. Washes with RIPA Buffer 4 times and once of TE with 50mM NaCl. Reversed crosslink and DNA purified for sequencing using phenol:chloroform:isoamyl alcohol.