GSM3937808: RPMI8226 DMSO 4 days Input ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
RPMI 8226
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma
Attributes by original data submitter
Sample
source_name
Multiple Myeloma
cell line
RPMI8226
treatment
DMSO 4 days
chip antibody target
Input
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were pelleted, fixed in 1% formaldehyde for 10 mins, lysed and sonicated. Chromatin samples were precleared with Protein A Dynabeads (Life Technologies), and incubated overnight at 40C with anti-EZH2 (Millipore 07-689), anti-H3K27me3 (Cell Signaling Techonology 9730), anti-SUZ12 (Active Motif 39357), and anti-H3K4me3 (Abcam ab8580). Chromatin-antibody complexes were precipitated using Protein A Dynabeads followed by washes in RIPA buffer and Tris/ EDTA. Samples were digested with RNAase A, treated with Proteinase K and 10% SDS, followed by cross-link reversal at 650C. DNA was purified using MinElute PCR purification kits (Qiagen). DNA libraries for ChIP-seq were prepared using Ovation Ultralow DR Multiplex System kits (0330-32, NuGEN) followed by Illumina sequencing.