Antigen

Antigen Class

TFs and others

Antigen

SUZ12

Cell type

Cell type Class

Blood

Cell type

KARPAS-422

Primary Tissue

Blood

Tissue Diagnosis

Lymphoma Non-Hodgkins

Sample

source_name

Diffuse large B cell lymphoma

cell line

KARPAS-422

treatment

1.5uM CPI360 4 days

chip antibody target

Suz12

chip antibody

Active Motif 39357

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, cells were pelleted, fixed in 1% formaldehyde for 10 mins, lysed and sonicated. Chromatin samples were precleared with Protein A Dynabeads (Life Technologies), and incubated overnight at 40C with anti-EZH2 (Millipore 07-689), anti-H3K27me3 (Cell Signaling Techonology 9730), anti-SUZ12 (Active Motif 39357), and anti-H3K4me3 (Abcam ab8580). Chromatin-antibody complexes were precipitated using Protein A Dynabeads followed by washes in RIPA buffer and Tris/ EDTA. Samples were digested with RNAase A, treated with Proteinase K and 10% SDS, followed by cross-link reversal at 650C. DNA was purified using MinElute PCR purification kits (Qiagen). DNA libraries for ChIP-seq were prepared using Ovation Ultralow DR Multiplex System kits (0330-32, NuGEN) followed by Illumina sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

31374399

Reads aligned (%)

85.7

Duplicates removed (%)

8.5

Number of peaks

1927 (qval < 1E-05)

hg38

Number of total reads

31374399

Reads aligned (%)

86.3

Duplicates removed (%)

7.5

Number of peaks

1953 (qval < 1E-05)