Antigen

Antigen Class

TFs and others

Antigen

TCF12

Cell type

Cell type Class

Liver

Cell type

Hep 3B

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma

Sample

source_name

Hep3B

cell line

Hep3B

cell type

Hepatocellular carcinoma cells

chip-antibody

CST11825, Danvers, MA, USA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

TCF12 antibody (CST11825, Danvers, MA, USA) and protein A magnetic beads were incubated with the lysate overnight at 4 °C and then reverse crosslinked. Libraries were prepared according to KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series- KK8504

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

18807797

Reads aligned (%)

67.2

Duplicates removed (%)

9.2

Number of peaks

881 (qval < 1E-05)

hg19

Number of total reads

18807797

Reads aligned (%)

65.2

Duplicates removed (%)

9.8

Number of peaks

420 (qval < 1E-05)