Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF12

Cell type

Cell type Class
Liver
Cell type
Hep 3B
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Hep3B
cell line
Hep3B
cell type
Hepatocellular carcinoma cells
chip-antibody
CST11825, Danvers, MA, USA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
TCF12 antibody (CST11825, Danvers, MA, USA) and protein A magnetic beads were incubated with the lysate overnight at 4 °C and then reverse crosslinked. Libraries were prepared according to KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series- KK8504

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
18807797
Reads aligned (%)
117.7
Duplicates removed (%)
9.8
Number of peaks
420 (qval < 1E-05)

hg38

Number of total reads
18807797
Reads aligned (%)
122.0
Duplicates removed (%)
9.2
Number of peaks
881 (qval < 1E-05)

Base call quality data from DBCLS SRA