Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Liver
Cell type
Hep 3B
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Hep3B
cell line
Hep3B
cell type
Hepatocellular carcinoma cells
chip-antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
TCF12 antibody (CST11825, Danvers, MA, USA) and protein A magnetic beads were incubated with the lysate overnight at 4 °C and then reverse crosslinked. Libraries were prepared according to KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series- KK8504

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
18025886
Reads aligned (%)
66.4
Duplicates removed (%)
7.9
Number of peaks
1189 (qval < 1E-05)

hg19

Number of total reads
18025886
Reads aligned (%)
63.3
Duplicates removed (%)
8.8
Number of peaks
513 (qval < 1E-05)

Base call quality data from DBCLS SRA