Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KAT7

Cell type

Cell type Class
Blood
Cell type
MOLM-13
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Human cell line
cell line
MOLM13
genotype
wildtype
chip antibody
anti-KAT7 (Abcam ab70183)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP samples were washed with RIPA wash buffer (50mM Hepes, 500mM LiCl, 1mM EDTA, 1% NP-40, 0.7% Na-Deoxycholate) 3 times followed by wash with Annealing buffer (TE+ 50mM NaCl). Elution Buffer (1% SDS, 50mM Tris-HCl, 10mM EDTA) was added to each ChIP samples and heated at 65°C for 30min. Beads were subsequently removed and samples were heated, at 14000rpm, 65°C and supplemented with 0.2 mg/mL RNase A (ThermoFisher). 0.2 mg/mL Proteinase K (Life Technologies) were added the next day to digest proteins by incubating shaking at 450rpm at 65°C for 4h. ChIP purified by PCR purification Kit (Qiagen). PBS and LB1, LB2, LB3 and RIPA wash buffers were all supplemented with Sodium Butyrate (Sigma), cOmplete EDTA-free protease inhibitor cocktail (Roche) and PMSF (Sigma) immediately before use. RNA was extracted from AML cells with RNeasy Plus Mini Kit (Qiagen) according to manufacturer's instructions. Sequencing was performed on Illumina HiSeq v4 platform with 75-bp paired-end sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
12018332
Reads aligned (%)
157.3
Duplicates removed (%)
13.2
Number of peaks
191 (qval < 1E-05)

hg38

Number of total reads
12018332
Reads aligned (%)
158.9
Duplicates removed (%)
13.0
Number of peaks
286 (qval < 1E-05)

Base call quality data from DBCLS SRA