Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Neural
Cell type
CLB-Ga
NA
NA

Attributes by original data submitter

Sample

source_name
CLB-GA
cell line
CLB-GA
cell type
Neuroblastoma
treatment
Dinaciclib, 10 nM, 8 h
chip antibody
Brd4 (A301-985A50, Lot # 6, BETHYL Laboratories)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as described previously (Lee TI, Johnstone SE, Young RA. Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc. 2006;1(2):729-748) ChIP-seq libraries were prepared according to the Illumina protocol for ChIP-seq

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
30390390
Reads aligned (%)
33.3
Duplicates removed (%)
65.5
Number of peaks
451 (qval < 1E-05)

hg38

Number of total reads
30390390
Reads aligned (%)
35.9
Duplicates removed (%)
63.3
Number of peaks
848 (qval < 1E-05)

Base call quality data from DBCLS SRA