Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Neural

Cell type

CLB-Ga

NA

NA

Sample

source_name

CLB-GA

cell line

CLB-GA

cell type

Neuroblastoma

treatment

Control No Treatment

chip antibody

Brd4 (A301-985A50,Lot # 6, BETHYL Laboratories)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) was performed as described previously (Lee TI, Johnstone SE, Young RA. Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc. 2006;1(2):729-748) ChIP-seq libraries were prepared according to the Illumina protocol for ChIP-seq

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

41010903

Reads aligned (%)

96.8

Duplicates removed (%)

67.5

Number of peaks

1100 (qval < 1E-05)

hg19

Number of total reads

41010903

Reads aligned (%)

95.3

Duplicates removed (%)

69.7

Number of peaks

723 (qval < 1E-05)