Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Neural
Cell type
CLB-Ga
NA
NA

Attributes by original data submitter

Sample

source_name
CLB-GA
cell line
CLB-GA
cell type
Neuroblastoma
treatment
Control No Treatment
chip antibody
Brd4 (A301-985A50,Lot # 6, BETHYL Laboratories)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as described previously (Lee TI, Johnstone SE, Young RA. Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc. 2006;1(2):729-748) ChIP-seq libraries were prepared according to the Illumina protocol for ChIP-seq

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
41010903
Reads aligned (%)
28.9
Duplicates removed (%)
69.7
Number of peaks
723 (qval < 1E-05)

hg38

Number of total reads
41010903
Reads aligned (%)
31.4
Duplicates removed (%)
67.5
Number of peaks
1100 (qval < 1E-05)

Base call quality data from DBCLS SRA