GSM3904401: TOP2B BT142 DMSO chip-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TOP2B
Cell type
Cell type Class
Neural
Cell type
BT142
NA
NA
Attributes by original data submitter
Sample
source_name
BT142 glioma cells
agent
DMSO
genotype
BT142
cell type
glioma cells
chip antibody
TOP2B
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear extracts were isolated, chromatin fragmented using Covaris sonicator in 1mL tubes for 1min. 10 ug of antibody (TOP2A Abcam TOP2B Abcam) wasc incubated overnight. Washes with RIPA Buffer 4 times and once of TE with 50mM NaCl. Reversed crosslink and DNA purified for sequencing using phenol:chloroform:isoamyl alcohol.