Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
human neural progenitor cells
cell line
H1
cell type
human neural progenitor cells
genotype
WT
chip antibody
anti-FLAG M2 (Sigma-Aldrich, Catalog number M8823)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, 1×10^7 cells of each sample were crosslinked in 1% formaldehyde with rotation for 10 minutes at room temperature. And then these crosslinked reactions were stopped by 0.125 M glycine with rotation for 5 minutes at room temperature. and cell sample were sonicated, and then ChIPed with FLAG M2-beads. DNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
130778798
Reads aligned (%)
90.7
Duplicates removed (%)
7.4
Number of peaks
2004 (qval < 1E-05)

hg38

Number of total reads
130778798
Reads aligned (%)
92.8
Duplicates removed (%)
6.0
Number of peaks
3077 (qval < 1E-05)

Base call quality data from DBCLS SRA