GSM1421318: Bre98p5 input ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
Mammary epithelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Human Mammary Epithelial Cell Line
tissue
Breast
passage
5
growth phase
HMEC
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments utilised ~2x10^6 cells (per immunoprecipitation) fixed for 10 minutes at 37°C in culture media containing 2.7% (w/v) formaldehyde (Sigma-Aldrich, St Louis MO, USA). Immunoprecipitation was performed using the Chromatin Immunoprecipitation Assay Kit (Merck Millipore, Billerica MA, USA) according to the manufacturers protocol withthe included salmon sperm DNA blocked agarose beads substituted with Protein A/G PLUS-Agarose Immunoprecipitation Reagent (Santa Cruz Biotechnology). Illumina 36 bp single end sequencing was performed on 5-12 ng of ChIP DNA. 10ng DNA of MBDCap enriched DNA was prepared for Ilumina sequencing using the Illumina ChIP-Seq DNA sample prep kit (IP-102-1001) according to the manufacturer’s instructions. The library preparation was analyzed on Agilent High Sensitivity DNA 1000 Chip.