Cells were fixed in 1% formaldehyde at room temperature for 10 min and Ikaros ChIP were performed with two different Ikaros Antibodies using protocol essentially as descrbed in O'Geen, H., Frietze, S. & Farnham, P.J. Using ChIP-seq technology to identify targets of zinc finger transcription factors. Methods Mol. Biol. 649, 437–455 (2010) Single-end multiplexed libraries were created essentially as described in O'Geen, H., Frietze, S. & Farnham, P.J. Using ChIP-seq technology to identify targets of zinc finger transcription factors. Methods Mol. Biol. 649, 437–455 (2010)