Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
IKZF1

Cell type

Cell type Class
Blood
Cell type
ICN1
NA
NA

Attributes by original data submitter

Sample

source_name
ICN1
cell type
Xenograft-derived BCR-ABL1+ (Ph+) human pre-B ALL
chip-ab
anti-Ikaros, clone H-100 (Santa Cruz, cat # sc-13039 X)
replicate
ICN1-replicate2 (H-100 Ab)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde at room temperature for 10 min and Ikaros ChIP were performed with two different Ikaros Antibodies using protocol essentially as descrbed in O'Geen, H., Frietze, S. & Farnham, P.J. Using ChIP-seq technology to identify targets of zinc finger transcription factors. Methods Mol. Biol. 649, 437–455 (2010) Single-end multiplexed libraries were created essentially as described in O'Geen, H., Frietze, S. & Farnham, P.J. Using ChIP-seq technology to identify targets of zinc finger transcription factors. Methods Mol. Biol. 649, 437–455 (2010)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
67973028
Reads aligned (%)
85.0
Duplicates removed (%)
5.6
Number of peaks
5269 (qval < 1E-05)

hg38

Number of total reads
67973028
Reads aligned (%)
87.3
Duplicates removed (%)
4.1
Number of peaks
5768 (qval < 1E-05)

Base call quality data from DBCLS SRA