GSM1420157: HCT116-H3K36me3 Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K36me3
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116 cells
cell line
HCT116
cell type
colon cancer
genotype
wildtype
Sex
male
chip antibody
anti-H3K36me3
antibody manufacturer
Abcam
antibody catalog number
9050
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed and nuclei were sonicated. Histone-DNA complexes were isolated with target antibody. For NOMe-seq samples, freshly isolated nuclei were treated with M.CviPI Libraries were prepared according to Illumina's recommendation from 20ng ChIP'ed DNA and indexed prior to sequencing on HiSeq2000 using 50SR cycles for ChIP-seq and 75PE for NOMe-seq samples