GSM1417250: HCT116 p53 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
colorectal cancer cell
Sequenced DNA Library
ChIP and Input DNA libraries for single end sequencing were prepared with the Illumina ChIP-Seq DNA Sample Prep kit according to the manufacturer’s instructions. DNA was purified using QIAGEN PCR clean up kit, DNA fragments were 3’ A-tailed and ligated to Illumina adapters. Adapter-ligated DNA was purified using Agencourt AMPure kit, and the entire amount of eluted DNA was used for PCR amplification (10-12 cycles). The PCR amplified ChIP and Input libraries were subjected to electrophoresis and fragments with average size ~200-400 bp were recovered using the QIAGEN gel extraction kit. Libraries was quantified on Agilent 2100 Bioanalyzer using the High Sensitivity DNA kit and single end sequencing was performed on Illumina Genome Analyzer 2x.