Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
F2RL1

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Human embryonic kidney cells
passages
15-18
cell line
HEK293
chip antibody
PAR2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and PAR2-DNA complexes were isolated with PAR2 antibody. Precipitated ChIP DNA was isolated GFX PCR DNA and Gel Band purifcation kit from Qiagen TruSeq ChIP Sample Preparation Kits

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
42380017
Reads aligned (%)
85.9
Duplicates removed (%)
4.0
Number of peaks
1022 (qval < 1E-05)

hg38

Number of total reads
42380017
Reads aligned (%)
87.7
Duplicates removed (%)
2.6
Number of peaks
738 (qval < 1E-05)

Base call quality data from DBCLS SRA