Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CSNK2A1

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
CK2α ChIP-seq
antibody
Anti- CK2α Abcam (Cat. No. Ab70774)
passages
40-45
cell type
Prostate Carcinoma Cells
cell line
LNCaP
treatment
100 nM DHT for two and a half hour

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
27931210
Reads aligned (%)
80.5
Duplicates removed (%)
10.3
Number of peaks
3279 (qval < 1E-05)

hg38

Number of total reads
27931210
Reads aligned (%)
81.7
Duplicates removed (%)
9.4
Number of peaks
3164 (qval < 1E-05)

Base call quality data from DBCLS SRA