Antigen

Antigen Class

TFs and others

Antigen

CSNK2A1

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

CK2α ChIP-seq

antibody

Anti- CK2α Abcam (Cat. No. Ab70774)

passages

40-45

cell type

Prostate Carcinoma Cells

cell line

LNCaP

treatment

100 nM DHT for two and a half hour

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

27931210

Reads aligned (%)

90.2

Duplicates removed (%)

9.4

Number of peaks

3164 (qval < 1E-05)

hg19

Number of total reads

27931210

Reads aligned (%)

89.7

Duplicates removed (%)

10.3

Number of peaks

3279 (qval < 1E-05)