GSM1415879: HCT116-H3K36me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K36me3
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116 cells
cell line
HCT116
cell type
colon cancer cell line
Sex
male
chip antibody
anti-H3K36me3 (Abcam #9050)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed and nuclei were sonicated. Histone-DNA complexes were isolated with target antibody. Libraries were prepared according to Illumina's recommendation from 20ng ChIP'ed DNA and indexed prior to sequencing on HiSeq2000 using 50SR cycles