Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

U-87 MG

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

Glioblastoma cells

cell line

U87-EGFRvIII

cell type

glioblastoma cells

RNAi treatment

Rictor

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared with SimpleChIPTM Enzymatic Chromatin IP Kit (CST, Beverly, MA) and histone-DNA complexes were isolated with antibody. Libraries were prepared according to instructions accompanying the KAPA library preparation kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

49811299

Reads aligned (%)

99.2

Duplicates removed (%)

4.6

Number of peaks

990 (qval < 1E-05)

hg19

Number of total reads

49811299

Reads aligned (%)

98.0

Duplicates removed (%)

6.5

Number of peaks

920 (qval < 1E-05)