Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MEN1

Cell type

Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Prostate cancer cells
cell line
PC-3
cell type
Derived from metastatic site: Bone
passages
18
source tissue
androgen resistant prostate cancer
chip antibody
rabbit anti-Menin polyclonal antibody (Bethyl, INC)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were generated using the MicroPlex Library Preparation Kit (Diagenode) following the manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
62227574
Reads aligned (%)
77.6
Duplicates removed (%)
20.5
Number of peaks
1339 (qval < 1E-05)

hg38

Number of total reads
62227574
Reads aligned (%)
79.7
Duplicates removed (%)
19.0
Number of peaks
1565 (qval < 1E-05)

Base call quality data from DBCLS SRA