Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA4

Cell type

Cell type Class
Digestive tract
Cell type
ESO-26
NA
NA

Attributes by original data submitter

Sample

source_name
Eso26_GATA4-Flag Chip-seq
cell line
ESO26
cell type
Esophageal Adenocarcinoma cell line
chip antibody
Anti-GATA4-Flag (Sigma; F1804)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Total RNA was extracted by RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. Standard Illumina library preparation protocol

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
9707224
Reads aligned (%)
159.0
Duplicates removed (%)
2.3
Number of peaks
463 (qval < 1E-05)

hg38

Number of total reads
9707224
Reads aligned (%)
160.8
Duplicates removed (%)
2.3
Number of peaks
555 (qval < 1E-05)

Base call quality data from DBCLS SRA