Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
SU-DIPG-36
NA
NA

Attributes by original data submitter

Sample

source_name
SU-DIPG36 patient-derived DIPG culture
tissue
Diffuse intrinsic pontine glioma (DIPG)
antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using PCI purification End-repair and A-tailing performed as described in Nagaraja 2017. Adaptor ligation and indexing with NEBNext Multiplex Oligos

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
21894171
Reads aligned (%)
98.2
Duplicates removed (%)
2.4
Number of peaks
769 (qval < 1E-05)

hg19

Number of total reads
21894171
Reads aligned (%)
97.4
Duplicates removed (%)
2.6
Number of peaks
478 (qval < 1E-05)

Base call quality data from DBCLS SRA