Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Immortal cell line

cell line

THP-1

antibody target

H3K27me3

primary antibody

mouse monoclonal anti-H3K27me3 (MABI0323, MAB Institute)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fixed cells were lysed and histone-DNA complexes were isolated with antibodies. Libraries were prepared on the Illumina Next-Seq 500 platform.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

31039474

Reads aligned (%)

89.5

Duplicates removed (%)

38.3

Number of peaks

32062 (qval < 1E-05)

hg19

Number of total reads

31039474

Reads aligned (%)

89.3

Duplicates removed (%)

39.0

Number of peaks

31742 (qval < 1E-05)