Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

cell line

Human K562 cells

cell type

immortalized myelogenous leukemia cells

chip antibody

Millipore, 07-729, Lot: 2971001

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq analysis using the Illumina NextSeq500, 10-20 ng of ChIP DNA was processed for library generation using the NEBNext ChIP-seq Library Prep Master Mix following the manufacturer's protocol (New England Biolabs). Raw reads that aligned to exactly one location in the reference human genome (hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

9222005

Reads aligned (%)

95.9

Duplicates removed (%)

32.6

Number of peaks

22073 (qval < 1E-05)

hg19

Number of total reads

9222005

Reads aligned (%)

95.2

Duplicates removed (%)

33.9

Number of peaks

21791 (qval < 1E-05)