Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat
cell line
Human Jurkat cells
cell type
immortalized T lymphocyte cells
chip antibody
Abcam, ab4729, Lot: GR3231937-1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq analysis using the Illumina NextSeq500, 10-20 ng of ChIP DNA was processed for library generation using the NEBNext ChIP-seq Library Prep Master Mix following the manufacturer's protocol (New England Biolabs). Raw reads that aligned to exactly one location in the reference human genome (hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
13239971
Reads aligned (%)
84.5
Duplicates removed (%)
6.2
Number of peaks
20322 (qval < 1E-05)

hg19

Number of total reads
13239971
Reads aligned (%)
83.9
Duplicates removed (%)
6.7
Number of peaks
20194 (qval < 1E-05)

Base call quality data from DBCLS SRA