Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTNNB1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Human embryonic stem cells
cell line
SA121
cell type
Human embryonic stem cells
treatement
CHIR99021+SB431542
chip antibody
anti-Beta-Catenin (R&D systems, AF1329)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed for 15 min in culture media containing 1% formaldehyde and were processed for ChIP as in Dietrich et al. (2007) EMBO J 26: 1637–1648. DNA from three parallel ChIPs were pooled and 10 ng was used for making ChIP-seq libraries. Libraries were generated according to Illumina recommendations and sequencing was done on a Genome Analyzer II (Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
73688107
Reads aligned (%)
34.8
Duplicates removed (%)
63.8
Number of peaks
1232 (qval < 1E-05)

hg38

Number of total reads
73688107
Reads aligned (%)
36.7
Duplicates removed (%)
62.2
Number of peaks
1240 (qval < 1E-05)

Base call quality data from DBCLS SRA