GSM1412243: CHIR SB BetaCatenin; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTNNB1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Human embryonic stem cells
cell line
SA121
cell type
Human embryonic stem cells
treatement
CHIR99021+SB431542
chip antibody
anti-Beta-Catenin (R&D systems, AF1329)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed for 15 min in culture media containing 1% formaldehyde and were processed for ChIP as in Dietrich et al. (2007) EMBO J 26: 1637–1648. DNA from three parallel ChIPs were pooled and 10 ng was used for making ChIP-seq libraries. Libraries were generated according to Illumina recommendations and sequencing was done on a Genome Analyzer II (Illumina).