Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TBP

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1
cell type
human embryonic stem cells
chip antibody
aTBP (Cell Signaling, 44059)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was digested by micrococcal nuclease protein-DNA complexes were isolated with aTBP (Cell Signaling, 44059). Chromatin was end-repaired, poly-adenylated, Y adaptor-ligated, and PCR-amplified.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
46071398
Reads aligned (%)
67.9
Duplicates removed (%)
29.8
Number of peaks
8896 (qval < 1E-05)

hg38

Number of total reads
46071398
Reads aligned (%)
68.7
Duplicates removed (%)
29.3
Number of peaks
9054 (qval < 1E-05)

Base call quality data from DBCLS SRA