Antigen

Antigen Class

TFs and others

Antigen

TBP

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

source_name

H1

cell type

human embryonic stem cells

chip antibody

aTBP (Cell Signaling, 44059)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was digested by micrococcal nuclease protein-DNA complexes were isolated with aTBP (Cell Signaling, 44059). Chromatin was end-repaired, poly-adenylated, Y adaptor-ligated, and PCR-amplified.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

46071398

Reads aligned (%)

97.1

Duplicates removed (%)

29.3

Number of peaks

9054 (qval < 1E-05)

hg19

Number of total reads

46071398

Reads aligned (%)

96.7

Duplicates removed (%)

29.8

Number of peaks

8896 (qval < 1E-05)