Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1
cell type
human embryonic stem cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was sheared by sonication and protein-DNA complexes were isolated with aK11 antibody (lab reagent). Sheared DNA was end-repaired, poly-adenylated, Y adaptor-ligated, and PCR-amplified.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
10225354
Reads aligned (%)
98.0
Duplicates removed (%)
2.8
Number of peaks
591 (qval < 1E-05)

hg19

Number of total reads
10225354
Reads aligned (%)
96.7
Duplicates removed (%)
3.7
Number of peaks
494 (qval < 1E-05)

Base call quality data from DBCLS SRA