Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MDA-MB-231 cell line

cell line

MDA-MB-231

cell type

breast cancer cell line

source tumor type

triple negative - basal b

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Fra-1 or Fra-2 complexes were isolated with the appropriate antibodies. ChIP-seq libraries were prepared using the TruSeq ChIP sample reparation kit from Illumina by the MGX sequencing Platform (Montpellier, France) ChIP-seq - Instrument model : Illumina HiSeq 2500 (MGX Platform, Montpellier France)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

92240880

Reads aligned (%)

97.8

Duplicates removed (%)

5.9

Number of peaks

1812 (qval < 1E-05)

hg19

Number of total reads

92240880

Reads aligned (%)

97.2

Duplicates removed (%)

7.5

Number of peaks

1582 (qval < 1E-05)