Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOSL1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231 cell line
cell line
MDA-MB-231
cell type
breast cancer cell line
source tumor type
triple negative - basal b
chip antibody
Fra-1 (sc-376148X, lot G0814, Santa Cruz Biotechnology)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Fra-1 or Fra-2 complexes were isolated with the appropriate antibodies. ChIP-seq libraries were prepared using the TruSeq ChIP sample reparation kit from Illumina by the MGX sequencing Platform (Montpellier, France) ChIP-seq - Instrument model : Illumina HiSeq 2500 (MGX Platform, Montpellier France)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
162769239
Reads aligned (%)
88.1
Duplicates removed (%)
8.9
Number of peaks
12292 (qval < 1E-05)

hg38

Number of total reads
162769239
Reads aligned (%)
90.5
Duplicates removed (%)
7.2
Number of peaks
13099 (qval < 1E-05)

Base call quality data from DBCLS SRA