Curated Sample Data


Genome
hg19
Antigen Class
Histone
Antigen
H3K27ac
Cell type Class
Blood
Cell type
Peripheral blood

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Peripheral blood
cell markers
N/A
disease
PLL
hdaci group
Resistant
treatment
N/A
chip antibody
H3K27ac (Abcam ab4729)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
0.05-0.5x106 cells were snap-frozen on dry ice for 15 minutes after 3 washes with PBS. Chromatin was prepared and ultra-low-input ChIP performed according to Brind'Amour et al., 2015 (Nature Communications). ChIP DNA was end-repaired, A-tailed, and ligated to Illumina PE adapters. Ligated fragments were PCR amplified using Illumina indexed primers for 12 cycles. DNA was purified using Ampure XP DNA purification beads between each step. Full methodology is available in Brind'Amour et al., 2015 (Nature Communications).

Platform Information


instrument_model
Illumina HiSeq 3000

External Database Query

Logs in read processing pipeline


Number of total reads
59073530
Reads aligned (%)
98.6
Duplicates removed (%)
19.7
Number of peaks
26009 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA