Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Acute myeloid leukemia

NA

NA

Sample

source_name

human AML cells

cell type

human t(8;21) AML cells

transduction

shcontrol

chip antibody

AML1-ETO (in-house made)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed using SimpleChIP® Enzymatic Chromatin IP Kits (Magnetic Beads, Cell Signaling Technology #9003) following the manufacturer's instructions. Libraries were constructed following instructions accompanying the library preparation kit designed for Illumina sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

14771903

Reads aligned (%)

97.5

Duplicates removed (%)

4.1

Number of peaks

6470 (qval < 1E-05)

hg19

Number of total reads

14771903

Reads aligned (%)

96.7

Duplicates removed (%)

5.0

Number of peaks

6462 (qval < 1E-05)