Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HDAC1

Cell type

Cell type Class
Blood
Cell type
Acute myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
human AML cells
cell type
human t(8;21) AML cells
transduction
AML1-ETO-specific shRNA
chip antibody
HDAC1 (Thermo, Fisher PA1-860)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using SimpleChIP® Enzymatic Chromatin IP Kits (Magnetic Beads, Cell Signaling Technology #9003) following the manufacturer's instructions. Libraries were constructed following instructions accompanying the library preparation kit designed for Illumina sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
29920231
Reads aligned (%)
77.4
Duplicates removed (%)
17.8
Number of peaks
716 (qval < 1E-05)

hg38

Number of total reads
29920231
Reads aligned (%)
79.4
Duplicates removed (%)
16.4
Number of peaks
796 (qval < 1E-05)

Base call quality data from DBCLS SRA