Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

Acute myeloid leukemia

NA

NA

Sample

source_name

human AML cells

cell type

human t(8;21) AML cells

transduction

shcontrol

chip antibody

N20 (Santa Cruz, sc-899)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed using SimpleChIP® Enzymatic Chromatin IP Kits (Magnetic Beads, Cell Signaling Technology #9003) following the manufacturer's instructions. Libraries were constructed following instructions accompanying the library preparation kit designed for Illumina sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

16788209

Reads aligned (%)

98.5

Duplicates removed (%)

5.2

Number of peaks

4789 (qval < 1E-05)

hg19

Number of total reads

16788209

Reads aligned (%)

97.6

Duplicates removed (%)

6.2

Number of peaks

4637 (qval < 1E-05)