In case of testicular cells, fixed cells were sorted using either FACS or MACS. Cells were sonicated in lysis buffer, and histone-DNA complexes were isolated from the cleared lysate using a-H3K9me3 antibody. ChIP-seq libraries were constructed using NEBNext ChIP-seq Library Prep Master Kit (NEB, E6240) and amplified using NEBNext Multiplex Oligos for Illumina (NEB, E7335). Final libraries were purified from 1.2% agarose gel - 200-450 bp fragments were excised. RNA-seq libraries were consucted using Illumina TruSeq RNA sample prep kit (v2, 48 rxns, RS-122-2001).