Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

miwi2HET_Liver_inputDNA

strain background

C57BL/6

age

post natal day 10

genotype/variation

Miwi2 Het

tissue/cell type

liver

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

In case of testicular cells, fixed cells were sorted using either FACS or MACS. Cells were sonicated in lysis buffer, and histone-DNA complexes were isolated from the cleared lysate using a-H3K9me3 antibody. ChIP-seq libraries were constructed using NEBNext ChIP-seq Library Prep Master Kit (NEB, E6240) and amplified using NEBNext Multiplex Oligos for Illumina (NEB, E7335). Final libraries were purified from 1.2% agarose gel - 200-450 bp fragments were excised. RNA-seq libraries were consucted using Illumina TruSeq RNA sample prep kit (v2, 48 rxns, RS-122-2001).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

13485463

Reads aligned (%)

98.6

Duplicates removed (%)

7.3

Number of peaks

355 (qval < 1E-05)

mm9

Number of total reads

13485463

Reads aligned (%)

98.4

Duplicates removed (%)

7.5

Number of peaks

356 (qval < 1E-05)