Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pluripotent stem cell
Cell type
EpiLC
NA
NA

Attributes by original data submitter

Sample

source_name
dY-H3K27ac ChIP
cell type
EpiLC (dY cells); epiblast-like cells
cell line of origin
dual miRNA reporter V6.5 ESCs
treatment
-Lif -2i day 3
chip antibody
H3K27ac
chip antibody vendor
Active Motif

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using a standard ChIP protocol Library was constructed using a standard Illumina protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
35340174
Reads aligned (%)
98.0
Duplicates removed (%)
19.6
Number of peaks
28386 (qval < 1E-05)

mm9

Number of total reads
35340174
Reads aligned (%)
97.8
Duplicates removed (%)
19.7
Number of peaks
28369 (qval < 1E-05)

Base call quality data from DBCLS SRA