GSM3814493: SMARCB1 ASO CTRL repl2 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMARCB1
Cell type
Cell type Class
Epidermis
Cell type
BJ
Primary Tissue
Skin
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
BJ hTERT ER:Ras cells
tissue
proliferating human fibroblasts
constructs
Stably expressing hTERT gene; inducible expression of ER:Ras
passage
less than 20 passages
treatment
ASO_CTRL transient transfection - cells collected after 36 hours
antibody
SNF5 - ChIP Grade (ab12167)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were double-crosslinked and nuclei were isolated. Lysates were clarified from sonicated nuclei and incubated with the corresponded antibody Antibody-DNA complexes were decrosslinked and DNA was isolated by SPRI beads. Libraries were prepared as previously described (Blecher-Gonen et al., 2013). Libraries were sequenced on the an Illumina Nextseq500 platform following the manufacturer's protocols.