Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Blood
Cell type
Multiple Myeloma
MeSH Description
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.

Attributes by original data submitter

Sample

source_name
TKO
parental cell line
KMS11
cell type
multiple myeloma cell line
genotype/variation
overexpressed NSD2 allele inactivated
kit
Nextera
chip antibody
Rad21 (Abcam ab992)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit. For ATAC-seq,ChIP-seq and HiChIP, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA libraries were prepared for sequencing using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines. RNAseq, ChIP-seq, ATAC-seq, Hi-C, HiChIP, 4C

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
54586758
Reads aligned (%)
34.9
Duplicates removed (%)
50.9
Number of peaks
2258 (qval < 1E-05)

hg38

Number of total reads
54586758
Reads aligned (%)
36.2
Duplicates removed (%)
49.6
Number of peaks
2468 (qval < 1E-05)

Base call quality data from DBCLS SRA