Antigen

Antigen Class

TFs and others

Antigen

RAD21

Cell type

Cell type Class

Blood

Cell type

Multiple Myeloma

MeSH Description

A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.

Sample

source_name

NTKO

parental cell line

KMS11

cell type

multiple myeloma cell line

genotype/variation

wild type NSD2 allele inactivated

kit

Nextera

chip antibody

Rad21 (Abcam ab992)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit. For ATAC-seq,ChIP-seq and HiChIP, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA libraries were prepared for sequencing using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines. RNAseq, ChIP-seq, ATAC-seq, Hi-C, HiChIP, 4C

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg19

Number of total reads

59156969

Reads aligned (%)

76.2

Duplicates removed (%)

63.0

Number of peaks

2102 (qval < 1E-05)

hg38

Number of total reads

59156969

Reads aligned (%)

76.9

Duplicates removed (%)

61.7

Number of peaks

2290 (qval < 1E-05)